Microbiological Activity of Mixed Disulfides for Lactobacillus fermenti.

Isolation and properties of mutants of Lactobacillus fermenti resistant to Amprolium.

A series of mutant strains of Lactobacillus fermenti resistant to the growth inhibitory properties of amprolium were isolated by serial transfers of the original strain in media which contained increasing concentrations of amprolium. Using three different resistance levels of strains (resistant strain A, B and C), the mechanism of amprolium resistance was investigated. Through the present studi...

Quantification of Global Protein Disulfides and Thiol-Protein Mixed Disulfides to Study the Protein Dethiolation Mechanisms

The redox state of cellular thiols is widely studied because it was recently linked to many different diseases and pathologies. In this work we quantified the concentrations of protein disulfides (PSSP) and thiol-protein mixed disulfides (XSSP) in rat tissues (liver, kidney and heart) and cells (Raw 264.7) by an improved method of XSSP and PSSP determination after oxidative stress induced by di...

Ak Improved Medium for Microbiological Assays with Lactobacillus Case1

Since its introduction as an assay organism for riboflavin (I), Lactobacilhs casei has been widely used for the determination of this and other vitamins which it requires (2-6). I,andy and Dicken (3) devised a single medium with which responses to each of t.he six vitamins required by this organism could be obtained. Experimental trial showed the medium to be lacking in substances which, though...

The microbiological estimation of Lactobacillus lactis Dorner activity with vitamin B12 as a standard.

Lactobacillus lactis Dorner has been reported to require two unidentified growth factors; one, a tomato juice component (TJ), and the other a component of liver extract, designated LLD (1, 2). Recently Shorb (3) and Rickes e2 al. (4) have shown that vitamin Blz was either wholly or partially responsible for the LLD activity of liver preparations. More recently Shive et al. (5) and Wright et al....

THE MICROBIOLOGICAL ASSAY OF VITAMIN Bu WITH LACTOBACILLUS LEICHMANNII